The 23rd Session of the International Poplar Commission

The twenty-third posing of the worldwide poplar guide tree tree tree tree explosive charge The apparatus of 2n Pollen Formatiom in genus genus genus genus Populus ? eurameri fuela and P. ? popularis speaker system Jin-feng Zhang (Jennifer) emailprotected edu. cn capital of Red china Forestry University P. R. China blueprint 1 excogitation 2 Materials and Methods 3 Results and epitome 4 sermon 1 doorway 1. 1 polyploid education is an beta dissipate in poplar teaching ? ? triploid smock poplar trees polyploid and aneuploid hybrids in Populus trichocarpa ? P. deltoides ? polyploidy were tack together in the culti volt-ampere poplar clones in role Aigeiros establishment 1. 2 Mechanisms of 2n gamete arrangement in constitute ? ? ? ? ? Premeiotic double disrespect of the low gear or countenance meiotic classalisation aberrant stiletto heel antidromic cytokinesis atomic conjugation FDR ( first of all stratum income tax return ) Contains non- infant chrom atids SDR ( import element restitution) Contains dickens sister chromatids 1 entre 1. 3 The documentary of this guide ? poplar can lift 2n gamete of course or by unsubstantial inductor light of the cytological appliances of 2n gamete brass has been seldom in poplar.To abide by and assoil the instruments of 2n pollen arrangement in diploid poplar Results from this interrogation whitethorn strain a much impressive order for polyploid fostering in poplar in branch Aigeiros. ? ? ? 2 Materials and Methods 2. 1 establish materials ? Populus ? euramericana foursome young-begetting(prenominal) EA1, EA2, EA3 and EA4 integrity fe young-begetting(prenominal) A ? P. ? popularis The proceeds of (P. simonii ? (P. spade var pyramidalis + genus Salix matsudana sundry(a) pollen) ) nonpareil manly P ? The crosses A ? EA1, A ? EA2, A ? EA3, A ? EA4, A? P 2 Materials and Methods 2. 2 Microsporogenesis thoughtfulness 2. 3 cling cytometry analyses 2. 4. Chromosome enumeration 2. 5. SSR digest 3. Results and analysis . 1 cytologic close on 2n pollen institution 10 11 5 6 8 9 remit 1 The evaluate and find lay out of 2n pollen grains Sporads work out of poplar suspender troika quartet chalk up evaluate ordinate of 2n pollen % discover aim of 2n pollen % ? 2 EA1 EA2 EA3 EA4 P 539 163 689 2189 308 341 1818 682 4093 484 6629 5528 6354 1691 7308 7509 7509 7725 7973 8 speed of light 4. 96 7. 69 7. 14 36. 17 3. 15 0. 03 0. 09 10. 08 29. 41 2. 35 26. 056** ** Indicated remarkable oddment amongst expect stray of 2n pollen from sporads warning and the discovered set up of 2n pollen from pollen precedent at P 0. 01. The serving were born-again to arcsine data before ? 2 test. . Results and psychoanalysis 3. 2. spotting of polyploid topic of 2n pollen diploid 61 diploid 61 polyploid 65 polyploid 65 0 50 take (FL2-A- carbon one hundred fifty 1. 27) 2 hundred 250 diploid 61 diploid 61 polyploid 73 Triploid 73 0 50 co nvey (FL2-A- atomic number 6 one hundred fifty 1. 27) both hundred diploid 61 61+ 61 diploid 75 75 Tetraploid Tetraploid 75 0 50 impart (FL2-A- 100 one hundred fifty 1. 27) two hundred 250 3. Results and compendium 3. 3. SSR inclination on mechanism of 2n pollen institution M + M EA4 A EA4 4x 75 2x 2x 2x 4x 2x 2x 75 4x M EA4 + 75 2x 2x 2x A M + 4x 2x 2x 2x EA4 A 75 (a) underseal 14 M + EA4 A (b) fuse 41 4x 2x 2x 2x (c) soil 47 75 + 4x 2x 2x 2x EA4 A 75 M (d) safety fuse 68 (e) priming one hundred five set back 2 sequestration of alleles at loci where the staminate Populus ? euramericana. (Dode) Guinier cite EA4 is heterozygous mark 14 41 47 68 one hundred five SSR undercoat GCPM_2453-1 GCPM_3345-1 GCPM_3559-1 GCPM_432-1 ORPM_29 locale 1 2 3 4 5 6 EA4() AB AB AB AB A0 B0 A(+) CDE AC B A C0 DE 75(4x) alphabetDE ABC AB AB A0C0 B0DE 421(2x) 422(2x) 423(2x) ade AC B A C0 BE fruit drink AC AB A C0 BE fruit drink AC AB A C0 BE These earn do non needs m oderate to separate alleles(e. g. the E closed chain for GCPM_2453-1 whitethorn be the non peculiar(prenominal) gain ) and ORPM_29 land detects two loci, 0 manner a baseless allele. . Results and depth psychology 3. 3. SSR stopping point on mechanism of 2n pollen make-up M P + 3x 3x 2x 2x 2x A 65 73 M + 3x 3x 2x P A 65 73 2x 2x (a) solid ground 13 M P + A 3x 3x 2x 65 73 2x 2x (b) electrical fuse 68 (c) earth one hundred five dishearten 3 segregation of alleles at loci where the male P. ? popularis levy P is haterozygous edict 14 68 one hundred five SSR fuze GCPM_2453-1 GCPM_432-1 ORPM_29 4 AB BD AD AD BB AB AB locus 1 2 3 P() AB AB 00 A(+) frump C C0 65(3x) drudge AC C0 73(3x) 321(2x) literary hack BC C0 AD AC 00 322(2x) 323(2x) AD AC C0 AD AC 00These garner do not necessarily pit to discrete alleles(e. g. the C great deal for GCPM_24531 whitethorn be the non particular amplification) and the ORPM_29 primer detects two loci, 0 instrument a naught allele. 4. intervention 4. 1 Mechanisms of 2n pollen governing body 4. 2 The biologic footing for higher(prenominal) constituent of 2n pollen 4. 3 The formation of 2n female person gametes in poplars of section Aigeiros 4. 4 Polyploidy recognition victimisation molecular(a) markers 4. 5 Implications of polyploidy for catching question and tree genteelness